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KMID : 0363620080290020007
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Journal of Korean Oriental Medicine
2008 Volume.29 No. 2 p.7 ~ p.20
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Effects of Kamijihwang-tang on Immune Cells and Cytokines in OVA-induced Asthmatic Mice
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Na Do-Gyun
Park Yang-Chun
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Abstract
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Objectives: The purpose of this study wasto examine the effects of Kamijihwang-tang (KJHT) extracts on immune cells and cytokines in ovalbumin (OVA)-induced asthmatic mice.
Methods: C57BL/6 mice were injected, inhaled and sprayed with OVA for 12 weeks (four times a week) for asthma induction. Two experimental groups were treated with different concentrations of KJHT (400 / and 200 / ) extracts and cyclosporine A (10 / ) for the later 8 weeks. At the end of the experiment, the mice lung, PLN and spleen were removed and immune cells were analyzed by flow cytometer, IL-5, IL-13, eotaxin-2, TNF-α were analyzed by real-time PCR, serum histamine was analyzed by ELISA kit.
Results: CD3+, CD3e-/CCR3+, CD3e+/CD69+, CD4+/CD25+, B220+/IgE+, and CD3e+/DX5+ cells in lung, PLN, and
spleen of the KJHT group (400 / ) decreased compared with that of the control group. CD3e+/CD69+,CD4+/CD25+, and CD3e+/DX5+ cells in lung, PLN, and spleen of the KJHT group (200 / ), CD3+, CD3e-/CCR3+ cells in lung and PLN of the KJHT group (200 / ) and B220+/IgE+ cells in lung and spleen of the KJHT group (200 / ) decreased compared with that of the control group. mRNA expression of IL-5, IL-13, eotaxin-2, and TNF- α in lung tissue of the KJHT groups (400 / and 200 / ) decreased compared with that of the control group. Histamine in serum of the KJHT group (400 / ) decreased compared with that of the control group.
Conclusions: These results suggest that KJHT can be utilized effectively in treating asthma because it significantly reduces inflammatory cells and cytokines.
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KEYWORD
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Asthma, Kamijihwang-tang (KJHT), immune cells, cytokine
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